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Why the effectiveness of biostimulants “floats”: analysis

Why the effectiveness of biostimulants “floats”: science, practice and the problem of trust

“My neighbor used the same product and got a 20% yield increase, while mine was almost zero.”

Such situations are well known to agronomists. They are often the reason for distrust of biostimulants, microbial preparations, and complex organo-mineral fertilizers.

However, a different result does not mean that the technology does not work. More often, it indicates something else: biological and organo-mineral products do not act in isolation, but within a complex system that includes the plant, soil, moisture, temperature, mineral nutrition, microbiota, and overall cultivation technology.

Therefore, the correct question is not:

“Do biostimulants work at all?”

And so:

“Under what conditions, on what crop, at what dose, and to solve what problem can a specific product provide the predicted result?”

What international research shows

One of the largest meta-analyses of the effectiveness of non-microbial biostimulants in field conditions included 1,087 paired observations from 180 studies.

The average yield increase was 17.9%. At the same time, significant statistical heterogeneity was recorded between the results of individual tests – I² at the level of 75% and more.

This means that the effect varied significantly depending on:

  • crops and varieties;
  • soil properties;
  • weather conditions;
  • method of application;
  • doses;
  • number of treatments;
  • phases of plant development;
  • protection against moisture and batteries.

Therefore, the average value of 17.9% should not be taken as a guaranteed increase for every field. It is a generalized result of a large number of experiments conducted under different conditions.

In the analyzed studies, a higher relative effect was more often observed:

  • in arid conditions;
  • on saline soils;
  • with low organic matter content;
  • in the absence of available power supplies;
  • on vegetable crops;
  • with properly selected soil application.

At the same time, the authors of the meta-analyses draw attention to the possible bias of scientific publications towards positive results. Successful trials are published more often than neutral or negative ones. Therefore, average indicators should be interpreted with caution and always correlated with specific farm conditions.

Author’s note — Timur LEVDA

“The natural system does not work on the principle of a button: you press it and always get the same result. We do not apply the product to an empty space, but to a specific soil with its own history, structure, water regime, and microbial life. Therefore, it is necessary to evaluate not only the product – you need to see the entire object.”

Why the results of biostimulants are different

The variation in results is not a random anomaly. It stems from the very nature of biostimulatory action.

1. Initial soil condition

The result is influenced by:

  1. organic matter content;
  2. soil structure and particle size distribution;
  3. acidity;
  4. salinization;
  5. availability of nitrogen, phosphorus, potassium and trace elements;
  6. the ability of the soil to retain water;
  7. soil microbiota activity;
  8. degree of compaction;
  9. history of the use of fertilizers and plant protection products.

In soil with sufficient nutrition, optimal structure, and high biological activity, the additional effect may be relatively small.

On degraded, low-humus, saline, compacted or arid soils, the potential for response is often greater, as the right product helps to alleviate a specific limiting factor.

2. Weather conditions

Many biostimulants manifest themselves most clearly under conditions of abiotic stress:

  • drought;
  • high or low temperatures;
  • sudden temperature fluctuations;
  • salinization;
  • violation of the water regime;
  • deficiency of certain nutrients.

In a favorable season, a plant can approach its genetic potential without additional stimulation. Therefore, the relative difference between the treated and control variants will be smaller.

But this does not necessarily mean that the biostimulant did not work. Its effect can manifest itself not only in tonnage, but also in:

  • root system development;
  • water use efficiency;
  • sowing uniformity;
  • resistance to short-term stress;
  • product quality;
  • soil microbiota activity;
  • better absorption of nutrients.

3. Culture, variety and development phase

Corn, wheat, vegetables, legumes, and berries have different physiological needs. Even two hybrids of the same crop may react differently to the same composition.

The time of application is equally important.

A product applied during the period of active root formation may give a different result than the same product applied after the main mass of roots has formed or after the onset of severe stress.

The biostimulant cannot fully compensate for:

  • late application;
  • critical deficiency of macronutrients;
  • errors in plant protection;
  • significant soil compaction;
  • prolonged lack of moisture;
  • incorrectly selected pH;
  • violation of crop rotation.

4. Method and dose of application

Soil, foliar, and seed application are different technological scenarios.

In international field studies, soil application has often shown a higher average effect than foliar or seed application. However, this does not mean that soil application will always be the best for every crop.

The result depends on:

  • warehouse;
  • solubility;
  • the ability of components to move in the soil;
  • mechanism of action;
  • target culture;
  • phases of development;
  • soil moisture.

Repeated applications also do not always automatically improve results. After a certain number of applications, the additional effect may decrease.

Therefore, the principle of “more inputs, better results” for biostimulants is not universal.

5. Product composition and standardization

Fundamentally different products can be sold under the general name “biostimulant”:

  • humic and fulvic substances;
  • seaweed extracts;
  • plant extracts;
  • amino acids and protein hydrolysates;
  • silicon compounds;
  • microbial consortia;
  • mineral carriers;
  • combined organo-mineral systems.

Even products in the same category can differ in:

  • origin of raw materials;
  • extraction method;
  • concentration of active ingredients;
  • purity;
  • stability;
  • compatibility of components;
  • storage conditions;
  • repeatability of composition between batches.

Therefore, the category name itself does not explain the mechanism of action and does not guarantee the same effectiveness.

Author’s note — Timur LEVDA

“Versatility should not mean the same formula for all conditions. Another principle is more correct: a single logic for building a product, but adapting it to the soil type, target pH, crop and environmental task.”

GREENODIN GRAY: not just a biostimulant, but a complex organo-mineral system

GREENODIN GRAY should be defined not simply as a biostimulant, but as a complex organo-mineral fertilizer with biostimulant components and predominantly soil action.

Its task is not only to short-term stimulate the physiological processes of the plant, but also to form a more favorable environment in the root system zone.

During the production of complex fertilizers TM GREENODIN, granules at the granulation stage are additionally enriched with a complex of biostimulating components:

  • auxins;
  • humic acids;
  • fulvic acids;
  • L-amino acids.

Each of these components performs its own function.

Auxins

Auxins are associated with the regulation of growth processes, cell division and elongation, root formation, and root system development.

Their presence in the complex product is aimed at maintaining active root formation, thanks to which the plant can use moisture and nutrients more efficiently.

Humic acids

Humic acids affect the physicochemical properties of the soil and the interaction between the soil matrix, roots, and nutrients.

They can contribute to:

  • improving soil structure;
  • moisture retention;
  • increasing buffering capacity;
  • activation of root processes;
  • increasing the availability of individual power supplies;
  • maintaining the biological activity of the rhizosphere.

Fulvic acids

Fulvic acids have a lower molecular weight and higher mobility compared to humic acids.

They can participate in the complexation and transport of nutrients, maintaining their availability to plants.

L-amino acids

L-amino acids are natural participants in plant metabolic processes. They are used in the synthesis of proteins, enzymes and other biologically active compounds.

Their application can support plant recovery under stressful conditions, especially when the synthesis of organic compounds requires significant energy expenditure.

Why are biostimulating components introduced at the granulation stage?

The introduction of auxins, humic acids, fulvic acids and L-amino acids at the granulation stage makes it possible to combine:

  1. mineral base;
  2. organic matrix;
  3. biostimulating substances;
  4. functional components of fertilizer

in one complex granular product.

In the soil, such a granule performs not only the function of a source of nutrients and minerals. It forms a local zone of interaction between:

  • root system;
  • soil moisture;
  • organic matter;
  • mineral components;
  • soil microbiota;
  • available batteries.

This fundamentally distinguishes a complex granular fertilizer from a product containing only one biostimulant or applied as a short-term foliar treatment.

At the same time, the presence of biostimulating components does not automatically mean the same increase in yield in all conditions.

The final result depends on:

  • cultures;
  • doses;
  • application phases;
  • soil properties;
  • moisture supply;
  • main power level;
  • weather conditions;
  • general cultivation technology.

Author’s note — Timur LEVDA

“At the granulation stage, we don’t just mechanically mix the ingredients. We form a functional granule in which the mineral base, organic matter, auxins, humic and fulvic acids, and L-amino acids must work as a single system.

The task of such fertilizer is not to stimulate the plant in the short term, but to create conditions around the root for nutrition, growth, moisture retention, and restoration of soil processes.”

What average performance indicators really mean

The results of different categories of biostimulants cannot be mechanically summarized in one table without specifying the indicator that was measured.

Some works evaluated:

  • marketable yield;
  • dry weight of roots and shoots;
  • length of the root system;
  • chlorophyll content;
  • stress resistance;
  • water use efficiency;
  • assimilation of nutrients;
  • quality of the final product.

Therefore, it is more correct to talk not about the abstract question of “which biostimulant is better”, but about the suitability of a specific product for a specific task.

What did the GREENODIN GRAY tests show?

GREENODIN GRAY field studies demonstrate the same general pattern as international meta-analyses: positive results were obtained in the studied variants, but their magnitude depended on the crop, dose, application time, and water regime.

Corn

On corn, hybrid DKC 3623, the application of GREENODIN GRAY at a dose of 250 kg/ha provided a yield increase of 1.29 t/ha compared to the control without the corresponding application.

The experimental version also recorded:

  • increasing the number of grains in a row by one grain;
  • increase in the mass of 1000 grains by 32.7 g.

The value of this result lies not only in the growth itself, but also in the clear indication of the crop, hybrid, dose, and control variant.

It is this level of detail that makes it possible to assess the conditions under which the result can be reproduced.

Winter garlic

In the study, the highest garlic yield — 15.3–15.9 t/ha — was obtained in variants with a dose of 300 kg/ha when planted in the third decade of October.

The result depended not only on the fertilizer itself, but also on:

  • doses;
  • planting time;
  • technological option;
  • water supply conditions.

The water consumption rate was lower than in the variant without appropriate fertilizer, by 16–38 m³ per ton of product.

The estimated profitability in the best options reached 139–140%.

Strawberry

The maximum yield of strawberries of the Rozana Kyivska variety — 66.2 c/ha — was recorded at a dose of GREENODIN GRAY of 500 kg/ha and maintaining soil moisture at 70–80–70% RH.

This example is particularly illustrative: the maximum effect was observed not from isolated fertilizer application, but from its combination with a proper moisture regime.

Additionally, a corresponding experiment recorded a decrease in the content of copper and zinc in berries by 23–30%.

This demonstrates that the result of using complex organo-mineral systems can be evaluated not only by yield, but also by product quality indicators.

These indicators characterize specific experiments and are not a promise of automatic repetition of identical results in every farm.

Author’s note — Timur LEVDA

“We do not consider GREENODIN GRAY as a substance that should give a one-time “push” to a plant. It is an element of the soil restoration system – its structure, moisture retention, mineral balance and living activity of the rhizosphere. Yield is an important, but not the only indicator of such restoration.”

Why two adjacent plots can give different results

Even neighboring fields do not necessarily have the same conditions.

They may differ in:

  • predecessor;
  • history of fertilizer and pesticide application;
  • sealing;
  • humus content;
  • acidity;
  • microrelief;
  • reserves of productive moisture;
  • the amount of plant residues;
  • the composition and activity of the microbiota;
  • depth of cultivation;
  • deadlines for field operations.

Therefore, the geographical proximity of the fields does not mean the same soil and technological conditions.

The result can also be influenced by:

  • date of entry;
  • soil moisture;
  • water quality;
  • compatibility with tank mix components;
  • temperature;
  • even distribution;
  • correct equipment settings;
  • method of wrapping granules in soil.

That is why a production experiment should include a control plot and, if possible, several replications.

From universal “insurance” to targeted technology

The main conclusion of scientific generalizations and our own field research is not that biostimulants are unpredictable.

The conclusion is different:

Predictability increases when a product is selected for a specific problem, specific soil, crop, development phase, and growing technology.

A biostimulant or complex fertilizer should not be purchased just because the advertisement states a certain average yield increase.

First, it is necessary to establish what factor is limiting the field’s productivity.

This could be:

  • insufficient root formation;
  • moisture deficiency;
  • low biological activity;
  • salinization;
  • disturbed pH;
  • poor availability of batteries;
  • low organic matter content;
  • consolidation;
  • post-stress recovery of plants.

Only after this can you assess whether the product’s composition and mechanism of action correspond to the real problem.

Author’s note — Timur LEVDA

“You shouldn’t impose a ready-made scheme on a natural object. First, you need to understand its condition and internal connections. Technology should not replace the natural process, but create conditions under which the soil and plants are once again able to realize their own potential.”

Agronomist’s checklist before purchasing

Before purchasing a biostimulant or a complex organo-mineral fertilizer, you should answer seven questions.

1. What field problem are we solving?

Not just “we want to increase yield”, but specifically:

  • drought;
  • salinization;
  • weak root system;
  • deficiency of organic matter;
  • pH disturbance;
  • low availability of batteries;
  • consolidation;
  • low biological activity.

2. Does the product’s mechanism of action address this problem?

You need to understand what exactly is included in the composition:

  • humic substances;
  • fulvic acids;
  • amino acids;
  • auxins;
  • microorganisms;
  • plant or algae extracts;
  • mineral components;
  • organic matrix.

3. Have tests been conducted on a similar culture?

Data on vegetable crops cannot be automatically transferred to grain crops, and the results of one hybrid cannot be automatically transferred to all hybrids.

4. How similar were the soil and climate?

Particularly important are:

  • • pH;
  • granulometric composition;
  • organic matter content;
  • salinization;
  • temperature;
  • moisture supply.

5. Are the dose and application method confirmed by field trials?

A laboratory result or a pot experiment does not guarantee similar effectiveness in the production field.

6. Will the control area be left?

Without control, it is impossible to reliably separate the effect of the product from the effects of weather, power, and other technological operations.

7. Will the technology be economically feasible under a conservative scenario?

The calculation should be made not based on the maximum advertising figure, but on the lower realistic limit of the expected effect.

FAQ

Does a large variation in results mean that biostimulants don’t work?

No. Meta-analyses demonstrate a positive average effect, but at the same time show significant dependence on crop, soil and climatic conditions, and application technology.

The average is not a guarantee of results on a particular field.

Why does the same product perform differently in neighboring fields?

Due to differences in:

  • soil structure;
  • organic matter content;
  • moisture reserves;
  • pH;
  • nutrition;
  • sealing;
  • microbiota;
  • predecessors;
  • deadlines and methods of payment.

On which soils can the effect be more pronounced?

According to international generalizations, a higher relative effect was more often observed:

  • in arid conditions;
  • on saline soils;
  • with low organic matter content;
  • due to a shortage of available power supplies.

However, this is a general statistical pattern, not a universal rule for every product.

Can a biostimulant replace the main fertilizer?

In most cases, no.

Biostimulants mainly supplement the nutrition system or increase resource efficiency, but cannot automatically compensate for critical macronutrient deficiencies.

Complex organo-mineral fertilizers, in particular GREENODIN GRAY, must be evaluated separately – according to their composition, content of nutritional and mineral components, and recommended application technology.

How to reduce the risk of a zero result?

Required:

  • conduct a soil analysis;
  • determine the limiting factor;
  • choose a product with an appropriate mechanism of action;
  • adhere to the recommended dose and application phase;
  • establish a control plot;
  • evaluate not only yield, but also root development, water consumption, product quality, and soil condition.

Main conclusion

The effectiveness of biostimulants is “floating” not because they are all random or ineffective.

It changes because the biostimulant becomes just one element of a complex biological and agrotechnological system.

International studies confirm an average positive effect, but at the same time demonstrate high variability in results.

GREENODIN GRAY’s own tests also show that it is not the abstract name of the product that matters most, but the right combination:

  • warehouse;
  • cultures;
  • doses;
  • payment deadline;
  • soil condition;
  • moisture supply;
  • general cultivation technology.

The inclusion of auxins, humic acids, fulvic acids and L-amino acids in the composition of TM GREENODIN fertilizers at the granulation stage makes it possible to form a complex functional granule.

However, even a complex granule cannot act independently of the real state of the soil and plant. Its effectiveness is revealed when the product is applied in a targeted manner, in the correct dose and as an element of a holistic technology.

Therefore, the future of the industry lies not in universal promises, but in:

  • diagnostics;
  • targeted application;
  • standardized field experiments;
  • transparent control;
  • economic calculation;
  • systematic soil restoration.

Final author’s position — Timur LEVDA

“Modern agriculture must learn not only to add substances, but also to manage the living conditions of the soil.”

“A strong technology is not one that forces a plant to produce more in a short period of time. It is a technology that simultaneously supports yield, water, soil structure, microbiome, and the ability of the agroecosystem to recover.”

Limitations of interpretation of results

The presented results of GREENODIN GRAY were obtained in specific experiments, under specific soil and climatic conditions, doses and cultivation technologies.

They demonstrate the effect recorded in the relevant experimental variants, but do not guarantee obtaining identical results in other farms.

Before large-scale application, it is recommended to conduct a local production test with a control area.

Sources

  1. A Meta-Analysis of Biostimulant Yield Effectiveness in Field Trials — Frontiers in Plant Science, 2022. https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.836702/full
  2. A Meta-Analysis of Biostimulant Yield Effectiveness in Field Trials — PMC. https://pmc.ncbi.nlm.nih.gov/articles/PMC9047501/
  3. Biostimulant yield effectiveness meta-analysis — New AG International, 2023. https://www.newaginternational.com/featurestrends/biostimulant-yield-effectiveness-meta-analysis/
  4. Biostimulants Market Share, Growth & Analysis Report — MarketDataForecast (дані метааналізу Wageningen University щодо водоростевих екстрактів). https://www.marketdataforecast.com/market-reports/biostimulants-market
  5. Humic field biostimulation as a sustainable agricultural practice to increase yield of main grains: evidence from on-farm trials — PMC, 2024. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12723518/
  6. Full article: Plant-biostimulants interaction: scientific trends, markets dynamics, and real-world implication — Taylor & Francis, 2025. https://www.tandfonline.com/doi/full/10.1080/17429145.2025.2572668
  7. Biostimulants for plants: types, mechanisms of action, possibilities — SuperAgronom.com. https://superagronom.com/articles/428-biostimulyatori-dlya-roslin-vidi-mehanizmi-diyi-mojlivosti-chi-potribni-biostimulyatori-kulturam
  8. Report on the implementation of scientific research “Substantiation of the use of complex organic fertilizer Greenodin gray on corn crops” — INSTITUTE OF NANOTECHNOLOGIES AND ORGANIC PRODUCTS “AVELIFE”.
  9. The effect of organo-mineral fertilizers on the development and yield of winter garlic plants — AVELIFE INSTITUTE.
  10. The effectiveness of organomineral fertilizers on yield and the possibility of obtaining environmentally friendly strawberry berries — AVELIFE INSTITUTE.

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